Appendices

Appendix A: List of PCR Primers
Appendix B Solution Recipes
Appendix C: Species Phylogeny
Appendix D: MsHSFA4 cDNA Sequence
Appendix E: MsHSFA4 Genomic Sequence
Appendix F: Fertilizer Nutrient Composition
Appendix G: DIG Probe Conformation of HSP 18 and HSP 86
Appendix H: Example of PCR Analysis of Transgenic Progeny
Appendix I: Design of the Chamber Experiment
Appendix J: Design of the Cultivar Field Experiment
Appendix K: Design of the Transgenic Experimental Blocks
Appendix L: Design of the Transgenic Field Experiment
Appendix M: Total Protein Accumulation during the Fall Field Experiment